Human PCBP1 antibody | NKMAX ATGA0284 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PCBP1 antibody

catalog :

ATGA0284

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2A10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PCBP1 antibody

Catalog number (SKU) :

ATGA0284

NCBI :

NP_006187

Description :

PCBP-1 and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. PCBP1 constitutes a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA.

Alternative Names :

Poly(rC) binding protein 1, Poly(rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24(10):1285-1292.;; Leffers H., et al. (1995) Eur J Biochem 230(2):447-53.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PCBP1 (1-163aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PCBP1 in HeLa cells. The cell was stained with ATGA0284 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of PCBP1 in MCF-7 cells. The cell was stained with ATGA0284 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0284-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate ;;Lane 2.: HepG2 cell lysate ;;Lane 3.: K562 cell lysate ;;Lane 4.: 293T cell lysate ;;Lane 5.: Jurkat cell lysate ;;Lane 6.: MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT2A10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15365

Internal Search Terms :

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more info or order :

NKMAX product webpage