Human PPM1F antibody | NKMAX ATGA0278 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PPM1F antibody

catalog :

ATGA0278

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6D11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PPM1F antibody

Catalog number (SKU) :

ATGA0278

NCBI :

NP_055449

Description :

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Alternative Names :

Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Koh, CG., et al. (2002) Curr Biol 12(4): 317-21.;; Tan, KM., et al. (2001) J Biol Chem 276(47): 44193-202.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PPM1F (1-454aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PPM1F in HeLa cells. The cell was stained with ATGA0278 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: PPM1F Transfected 293T cell lysate {ATGA0278-WB2.jpg}

Reacts With :

Human

Clone Number :

AT6D11

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P49593

Internal Search Terms :

PPM1F,ATGA0278-10ug,ATGA0278-20ug,ATGA0278-50ug,ATGA0278-100ug,ATGA0278-250ug,ATGA0278-500ug,ATGA0278-1mg,ATGA0278-10,ATGA0278-20,ATGA0278-50,ATGA0278-100,ATGA0278-250,ATGA0278-500,ATGA0278-1

more info or order :

NKMAX product webpage