Human Enolase 2/ENO2 antibody | NKMAX ATGA0273 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Enolase 2/ENO2 antibody

catalog :

ATGA0273

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT17D10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Enolase 2/ENO2 antibody

Catalog number (SKU) :

ATGA0273

NCBI :

NP_001966

Description :

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma.

Alternative Names :

Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Oliva D, Cali L, Feo S, Giallongo A (1991). Genomics 10 (1): 157-65.;; Craig SP, Day IN, Thompson RJ, Craig IW (1991). Cytogenet. Cell Genet. 54 (1-2): 71-3.;; McAleese SM, Dunbar B, Fothergill JE, et al. (1989). Eur. J. Biochem. 178 (2): 413-7.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human NSE (1-434aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of NSE in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NSE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: NSE Transfected 293T cell lysate {ATGA0273-WB2.jpg} The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: U-87 MG cell lysate ;;Lane 2.: Jurkat cell lysate {ATGA0273-WB3.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant protein ENO1 ;;Lane 2.: Recombinant protein NSE (ENO2) ;;Lane 3.: Recombinant protein ENO3 {ATGA0273-WB4.jpg}

Reacts With :

Human

Clone Number :

AT17D10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P09104

Internal Search Terms :

Enolase2, Neuron-specific Enolase, Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.,ATGA0273-10ug,ATGA0273-20ug,ATGA0273-50ug,ATGA0273-100ug,ATGA0273-250ug,ATGA0273-500ug,ATGA0273-1mg,ATGA0273-10,ATGA0273-20,ATGA0273-50,ATGA0273-100,ATGA0273-250,ATGA0273-500,ATGA0273-1

more info or order :

NKMAX product webpage