Human NBL1 antibody | NKMAX ATGA0267 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NBL1 antibody

catalog :

ATGA0267

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT38G8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NBL1 antibody

Catalog number (SKU) :

ATGA0267

NCBI :

NP_005371

Description :

NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development and alternatively spliced transcript variants have been identified for this gene. Also, as a tumor suppressor gene of neuroblastoma may play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 have been observed.

Alternative Names :

Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Olakowski, M., et al. (2009) Folia Histochem Cytobiol 47(2): 249-55.;; Shaikhibrahim, Z., et al. (2011) Int J Mol Med 28(4): 605-11.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NBL1 (18-181aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of NBL1 in Jurkat cells. The cell was stained with ATGA0267 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of NBL1 in A549 cells. The cell was stained with ATGA0267 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010222533296.jpg}

WB Comment and Pic :

The cell lysates(30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Jurkat cell lysate;;

Reacts With :

Human

Clone Number :

AT38G8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NBL1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P41271

Internal Search Terms :

DAN, NBL1,ATGA0267-10ug,ATGA0267-20ug,ATGA0267-50ug,ATGA0267-100ug,ATGA0267-250ug,ATGA0267-500ug,ATGA0267-1mg,ATGA0267-10,ATGA0267-20,ATGA0267-50,ATGA0267-100,ATGA0267-250,ATGA0267-500,ATGA0267-1

more info or order :

NKMAX product webpage