Human SMAC/Diablo antibody | NKMAX ATGA0266 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human SMAC/Diablo antibody

catalog :

ATGA0266

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT19F2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human SMAC/Diablo antibody

Catalog number (SKU) :

ATGA0266

NCBI :

NP_063940

Description :

SMAC/Diablo is one of the proapoptotic proteins. SMAC promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. In healthy cells, SMAC is a mitochondrial protein, but when cells undergo apoptosis, SMAC is released into the cytosol. SMAC is ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes

Alternative Names :

Diablo isoform 1, SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

McNeish. I.A., et al. (2003) Exp Cell Res 286(2): 186-198.;; Yu. J., et al. (2007) Oncogene 26(29): 4189-4198.;; Vucic. D., et al. (2002) J Biol Chem 277(14): 12275-12279.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human SMAC/Diablo (56-239aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of SMAC in HeLa cells. The cell was stained with ATGA0266 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: A431 cell lysate;;Lane 3.: Hep3B cell lysate;;Lane 4.: MCF7 cell lysate {ATGA0266-WB2.jpg}

Reacts With :

Human

Clone Number :

AT19F2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of SMAC in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NR28

Internal Search Terms :

SMADiablo,ATGA0266-10ug,ATGA0266-20ug,ATGA0266-50ug,ATGA0266-100ug,ATGA0266-250ug,ATGA0266-500ug,ATGA0266-1mg,ATGA0266-10,ATGA0266-20,ATGA0266-50,ATGA0266-100,ATGA0266-250,ATGA0266-500,ATGA0266-1

more info or order :

NKMAX product webpage