Human Cathepsin S antibody | NKMAX ATGA0264 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cathepsin S antibody

catalog :

ATGA0264

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1F9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cathepsin S antibody

Catalog number (SKU) :

ATGA0264

NCBI :

NP_004070

Description :

CTSS (Cathepsin S) is a lysosomal enzyme that belongs to the papain family of cysteine proteases. This protein is expressed by antigen presenting cells including macrophages, B-lymphocytes, dendritic cells and microglia. Moreover, CTSS is expressed in some epithelial cells. In contrast, cortical thymic epithelial cells do not express CTSS. Normal epidermal keratinocytes do not express CTSS. However, level of CTSS is elevated in psoriatic keratinocytes due to stimulation by proinflammatory factors. CTSS can function as an elastase over a broad pH range in alveolar macrophages.

Alternative Names :

Cathepsin S, MGC3886

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shi. G.P., et al. (1994) J Biol Chem 269: 11530-11536.;; Lemere. C.A., et al. (1995) Am J Pathol 146(4): 848-860.;; Shi. G.P., et al. (1992) J Biol Chem 267(11): 7258-7262.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CTSS (17-331aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of CTSS in U87MG cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of CTSS in HeLa cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0264-IF2.jpg}ICC/IF analysis of CTSS in A549 cells. The cell was stained with ATGA0264 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0264-IF3.jpg}

WB Comment and Pic :

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant Human CTSB ;;Lane 2.: Recombinant Human CTSD ;;Lane 3.: Recombinant Human CTSE ;;Lane 4.: Recombinant Human CTSF ;;Lane 5.: Recombinant Human CTSH ;;Lane 6.: Recombinant Human CTSK ;;Lane 7.: Recombinant Human CTSL ;;Lane 8.: Recombinant Human CTSS ;;Lane 9.: Recombinant Human CTSW ;;Lane 10.: Recombinant Human CTSZ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: U87MG cell lysate;;Lane 3.: A549 cell lysate {ATGA0264-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1F9

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P25774

Internal Search Terms :

Cathepsin S, CTSS,ATGA0264-10ug,ATGA0264-20ug,ATGA0264-50ug,ATGA0264-100ug,ATGA0264-250ug,ATGA0264-500ug,ATGA0264-1mg,ATGA0264-10,ATGA0264-20,ATGA0264-50,ATGA0264-100,ATGA0264-250,ATGA0264-500,ATGA0264-1

more info or order :

NKMAX product webpage