Human ICT1 antibody | NKMAX ATGA0263 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ICT1 antibody

catalog :

ATGA0263

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1E9

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ICT1 antibody

Catalog number (SKU) :

ATGA0263

NCBI :

NP_001536

Description :

Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.

Alternative Names :

Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Richter R., et al. (2010) EMBO J. 29:1116-1125.;;van Belzen N., et al. (1995) Eur. J. Biochem. 234:843-848.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ICT1 (30-206aa) purified from E. coli

Applications :

ELISA,WB,FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: ICT Transfected 293T cell lysate {ATGA0263-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1E9

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ICT1 in A549 cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q14197

Internal Search Terms :

ICT1,ATGA0263-10ug,ATGA0263-20ug,ATGA0263-50ug,ATGA0263-100ug,ATGA0263-250ug,ATGA0263-500ug,ATGA0263-1mg,ATGA0263-10,ATGA0263-20,ATGA0263-50,ATGA0263-100,ATGA0263-250,ATGA0263-500,ATGA0263-1

more info or order :

NKMAX product webpage