Human CNBP antibody | NKMAX ATGA0262 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CNBP antibody

catalog :

ATGA0262

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT38F10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CNBP antibody

Catalog number (SKU) :

ATGA0262

NCBI :

NP_001120668

Description :

CNBP is a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. It functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2.

Alternative Names :

Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

McGrath CF, Buckman JS, et al. (2003). J Virol. 77(15):8524-31.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CNBP (1-170aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CNBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0262-IF1b.jpg} ICC/IF analysis of CNBP in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CNBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0262-IF2a.jpg} {ATGA0262-IF2b.jpg}

WB Comment and Pic :

The cell lysate of 293T (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CNBP antibody (1:250 - 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT38F10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1000. Recommended starting dilution is 1:1000.

FACS Comment and Pic :

Flow cytometry analysis of CNBP in MCF7 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P62633

Internal Search Terms :

CNBP, Cellular nucleic acid-binding protein, CNBP1, DM2, PROMM, RNF163, ZCCHC22, ZNF9,ATGA0262-10ug,ATGA0262-20ug,ATGA0262-50ug,ATGA0262-100ug,ATGA0262-250ug,ATGA0262-500ug,ATGA0262-1mg,ATGA0262-10,ATGA0262-20,ATGA0262-50,ATGA0262-100,ATGA0262-250,ATGA0262-500,ATGA0262-1

more info or order :

NKMAX product webpage