Human CTLA4 antibody | NKMAX ATGA0260 product information

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product summary

company name :

NKMAX

product type :

antibody

product name :

Human CTLA4 antibody

catalog :

ATGA0260

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4C8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

product information

Product Type :

Antibodies

Item Name :

Human CTLA4 antibody

Catalog number (SKU) :

ATGA0260

NCBI :

NP_005205

Description :

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.

Alternative Names :

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dariavach. P., et al. (1988) Eur J Immunol 18(12): 1901-1905.;; Waterhouse. P., et al. (1995) Science 270(5238): 985-988.;; Magistrelli. G., et al. (1999) Eur J Immunol 29(11): 3596-3602.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CTLA-4 (36-161aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CTLA-4 in Jurkat cells. The cell was stained with ATGA0260 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA-4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse liver tissue lysate;; Lane 2.: TF-1 cell lysate;; Lane 3.: HepG2 cell lysate;; Lane 4.: WiDr cell lysate;;

Reacts With :

Human

Clone Number :

AT4C8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CTLA-4 in HeLa cells. The cell was stained with ATGA0260 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Stem cell

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P16410

Internal Search Terms :

CTLA4,ATGA0260-10ug,ATGA0260-20ug,ATGA0260-50ug,ATGA0260-100ug,ATGA0260-250ug,ATGA0260-500ug,ATGA0260-1mg,ATGA0260-10,ATGA0260-20,ATGA0260-50,ATGA0260-100,ATGA0260-250,ATGA0260-500,ATGA0260-1