Human Hexosaminidase A/HEXA antibody | NKMAX ATGA0259 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Hexosaminidase A/HEXA antibody

catalog :

ATGA0259

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT20F1

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Hexosaminidase A/HEXA antibody

Catalog number (SKU) :

ATGA0259

NCBI :

NP_000511.1

Description :

HEXA (Hexosaminidase A), also designated beta-Hexosaminidase A, is responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. A mutation in the a subunit of hexosaminidase is the cause of Tay-Sachs disease (TSD), also known as GM2-gangliosidosis type I. TSD is a fatal autosomal recessive lysosomal storage disease of the central nervous system (CNS) caused by insufficient activity of the HEXA enzyme that results in a failure to process GM2 gangliosides. The accumulation of GM2 ganglioside in the absence of HEXA activity causes progressive destruction of the CNS.

Alternative Names :

beta-hexosaminidase subunit alpha, TSD, hexosaminidase A

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cabezas. J.A. (1989) Biochem J 261(3): 1059-1060.;;Lemieux. M.J., et al. (2006) J Mol Biol 359(4): 913-929.;;Boles. D.J. and Proia. R.L. (1995) Am J Hum Genet 56(3): 716-724.

Abs Host :

Mouse

Isotype :

IgG2a Lambda

Immunogen :

Recombinant human HEXA (89-529aa) purified from E. coli

Applications :

ELISA,WB,FACS

WB Comment and Pic :

The cell lysate of MCF7 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: HEXA Transfected 293T cell lysate {ATGA0259-WB2.jpg}

Reacts With :

Human

Clone Number :

AT20F1

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of HEXA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P06865

Internal Search Terms :

Hexosaminidase A, HEXA,ATGA0259-10ug,ATGA0259-20ug,ATGA0259-50ug,ATGA0259-100ug,ATGA0259-250ug,ATGA0259-500ug,ATGA0259-1mg,ATGA0259-10,ATGA0259-20,ATGA0259-50,ATGA0259-100,ATGA0259-250,ATGA0259-500,ATGA0259-1

more info or order :

NKMAX product webpage