Human TDG antibody | NKMAX ATGA0251 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TDG antibody

catalog :

ATGA0251

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TDG antibody

Catalog number (SKU) :

ATGA0251

NCBI :

AAH37557

Description :

G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. Several bacterial proteins have strong sequence homology with this protein. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Alternative Names :

Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase,

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Neddermann, P ., et al. (1996) J Biol Chem 271(22): 12767-74;; Sard, L., et al. (1997) Genomics 44(2): 222-6;; Gallinari, P., et al. (1996) Nature 383(6602): 735-8

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human TDG (1-410aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of TDG in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TDG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0251-IF1b.jpg}

WB Comment and Pic :

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDG antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT2F7

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q13569

Internal Search Terms :

TDG, Thymine-DNA glycosylase, G/T mismatch-specific thymine DNA glycosylase,ATGA0251-10ug,ATGA0251-20ug,ATGA0251-50ug,ATGA0251-100ug,ATGA0251-250ug,ATGA0251-500ug,ATGA0251-1mg,ATGA0251-10,ATGA0251-20,ATGA0251-50,ATGA0251-100,ATGA0251-250,ATGA0251-500,ATGA0251-1

more info or order :

NKMAX product webpage