Human ADI1 antibody | NKMAX ATGA0248 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ADI1 antibody

catalog :

ATGA0248

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT27E8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ADI1 antibody

Catalog number (SKU) :

ATGA0248

NCBI :

NP_060739.1

Description :

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14.

Alternative Names :

Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gotoh I, Uekita T, Seiki M (2007). Genes Cells 12 (1): 105-17.;; Oram SW, Ai J, Pagani GM, et al. (2007). Neoplasia 9 (8): 643-51.;; Yeh CT, Lai HY, Chen TC, et al. (2001). J. Virol. 75 (22): 11017-24.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ADI1 (1-179aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of ADI1 in Hep3B cells. The cell was stained with ATGA0248 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADI1 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;

Reacts With :

Human

Clone Number :

AT27E8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9BV57

Internal Search Terms :

ADI1,ATGA0248-10ug,ATGA0248-20ug,ATGA0248-50ug,ATGA0248-100ug,ATGA0248-250ug,ATGA0248-500ug,ATGA0248-1mg,ATGA0248-10,ATGA0248-20,ATGA0248-50,ATGA0248-100,ATGA0248-250,ATGA0248-500,ATGA0248-1

References with this Product :

Bae DH, et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. (Biochim Biophys Acta Mol Basis Dis. 2020){https://pubmed.ncbi.nlm.nih.gov/32480040/}

more info or order :

NKMAX product webpage