Human MafK antibody | NKMAX ATGA0246 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human MafK antibody

catalog :

ATGA0246

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F7

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human MafK antibody

Catalog number (SKU) :

ATGA0246

NCBI :

NP_002351

Description :

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Alternative Names :

Transcription factor MafK, NFE2u, P18

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Oyake, T., et al. (1996) Mol Cell Biol 16(11): 6083-95.;; Toki, T., et al. (1997) Oncogene 14: 1901-1910.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human MAFK (1-156aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysate of Jurkat (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : Recombinant Human MAFK;;Lane 2. : Recombinant Human MAFG;;Lane 3. : Recombinant Human MAFF {ATGA0246-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2F7

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O60675

Internal Search Terms :

MafK,ATGA0246-10ug,ATGA0246-20ug,ATGA0246-50ug,ATGA0246-100ug,ATGA0246-250ug,ATGA0246-500ug,ATGA0246-1mg,ATGA0246-10,ATGA0246-20,ATGA0246-50,ATGA0246-100,ATGA0246-250,ATGA0246-500,ATGA0246-1

more info or order :

NKMAX product webpage