Human PDZK1 antibody | NKMAX ATGA0243 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PDZK1 antibody

catalog :

ATGA0243

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PDZK1 antibody

Catalog number (SKU) :

ATGA0243

NCBI :

NP_001188254

Description :

PDZK1 is PDZ domain-containing scaffolding protein with a 519 amino acid protein. PDZK1 mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. This protein is expressed in the kidney, pancreas, liver, gastrointestinal tract, and adrenal cortex. In situ hybridization experiments showed that the expression of PDZK1 was limited to epithelial cells. In addition, PDZK1 was overexpressed in selected tumors of epithelial origin.

Alternative Names :

Na(+)/H(+) exchange regulatory cofactor NHE-RF3, Na(+)/H(+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kocher, O., et al. (1999) Lab Invest 64: 1733-1745.;; Kocher, O., et al. (2001) Mol Cell Biol 23: 1175-1180.;; Gisler, S.M., et al (2003) Kidney Int 64(5): 1733-45.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human PDZK1 (1-519aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of PDKZ1 in Hep3B cells. The cell was stained with ATGA0243 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of HeLa (40ug) and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDZK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT1A2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PDZK1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q5T2W1

Internal Search Terms :

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more info or order :

NKMAX product webpage