Human CISD1 antibody | NKMAX ATGA0242 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CISD1 antibody

catalog :

ATGA0242

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A8

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CISD1 antibody

Catalog number (SKU) :

ATGA0242

NCBI :

NP_060934

Description :

CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.

Alternative Names :

DGSH iron-sulfur domain-containing protein 1, Cysteine transaminase CISD1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wiley, S.E., et al. (2007) J Biol Chem 282: 23745-23749.;; Paddock, M.L., et al. (2007) Proc Natl Acad Sci USA 104: 14342-14347.;; Taminelli, G.L., et al. (2008) Biochem Biophys Res Commun 365: 856-862.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CISD1 (32-108aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The tissue lysate of Mouse liver (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: K562 cell lysate;;Lane 2.: MCF7 cell lysate {ATGA0242-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1A8

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CISD1 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NZ45

Internal Search Terms :

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References with this Product :

He QQ, et al. MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection. (Sci Rep. 2016){https://pubmed.ncbi.nlm.nih.gov/27748416/}

more info or order :

NKMAX product webpage