Human N-Acetyl-D-Glucosamine Kinase/NAGK antibody | NKMAX ATGA0232 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human N-Acetyl-D-Glucosamine Kinase/NAGK antibody

catalog :

ATGA0232

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4D12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human N-Acetyl-D-Glucosamine Kinase/NAGK antibody

Catalog number (SKU) :

ATGA0232

NCBI :

NP_060037.2

Description :

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P (II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.

Alternative Names :

N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Weihofen WA, Berger M, Chen H, et al. (2007). J. Mol. Biol. 364 (3): 388-99.;; Weidanz JA, Campbell P, Moore D, et al. (1997). Br. J. Haematol. 95 (4): 645-53.;; Hinderlich S., et al. (Jul 2000). Eur J Biochem 267 (11): 3301-8.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NAGK (1-344aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of NAGK in HeLa cells. The cell was stained with ATGA0232 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane a.: 293T cell lysate.;;Lane b.: HeLa cell lysate.

Reacts With :

Human

Clone Number :

AT4D12

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UJ70

Internal Search Terms :

NAGK,ATGA0232-10ug,ATGA0232-20ug,ATGA0232-50ug,ATGA0232-100ug,ATGA0232-250ug,ATGA0232-500ug,ATGA0232-1mg,ATGA0232-10,ATGA0232-20,ATGA0232-50,ATGA0232-100,ATGA0232-250,ATGA0232-500,ATGA0232-1

more info or order :

NKMAX product webpage