Human GCLM antibody | NKMAX ATGA0229 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GCLM antibody

catalog :

ATGA0229

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2D12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GCLM antibody

Catalog number (SKU) :

ATGA0229

NCBI :

NP_002052

Description :

GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.

Alternative Names :

Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dickinson D.A., et al. (2005) Free Radic Biol Med 37(8): 1152-1159.;; Inoue Y., et al. (2003) Int J Oncol 23(5): 1333-1339.;; Robertson N.G., et al. (1995) Genomics 23(1): 42-50.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GCLM (1-274aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of GCLM in HeLa cells. The cell was stained with ATGA0229 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of HeLa (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT2D12

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P48507

Internal Search Terms :

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more info or order :

NKMAX product webpage