Human PIG3/TP53I3 antibody | NKMAX ATGA0227 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PIG3/TP53I3 antibody

catalog :

ATGA0227

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1C9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PIG3/TP53I3 antibody

Catalog number (SKU) :

ATGA0227

NCBI :

NP_671713

Description :

TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1, 2-naphtoquinone over 1, 4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.

Alternative Names :

Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Porte S, et al. (2009) J Biol Chem. 284(25):17194-205. ;; Flatt PM. et al. (2000) Cancer Lett. 156(1):63-72.;; J Biol Chem. (2009 Jun 19) 284(25):17194-205.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human TP53I3 (1-332aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of TP53I3 in A549 cells. The cell was stained with ATGA0227 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : A549 cell lysate ;;Lane 2. : SW480 cell lysate The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: TP53I3 Transfected 293T cell lysate {ATGA0227-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1C9

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q53FA7

Internal Search Terms :

NADPH quinone reductase PIG3, NADPH:quinone reductase PIG3, p53 induced gene 3 protein, p53-induced gene 3 protein, PIG3, Putative quinone oxidoreductase, Quinone oxidoreductase homolog, Quinone oxidoreductase PIG3, Quinone oxidoreductase PIG3 p53 induced gene 3 protein, TP53I3, Tumor protein p53 inducible protein 3, Tumor protein p53-inducible protein 3, ATGA0227-10ug, ATGA0227-20ug, ATGA0227-50ug, ATGA0227-100ug, ATGA0227-250ug, ATGA0227-500ug, ATGA0227-1mg, ATGA0227-010, ATGA0227-020, ATGA0227-050, ATGA0227-100, ATGA0227-250, ATGA0227-500, ATGA0227-01M

more info or order :

NKMAX product webpage