Human KRT23 antibody | NKMAX ATGA0225 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human KRT23 antibody

catalog :

ATGA0225

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human KRT23 antibody

Catalog number (SKU) :

ATGA0225

NCBI :

NP_056330

Description :

KRT23, also known as cytokeratin 23, is a 422 amino acid intermediate filament protein. The human KRT23 gene is located on chromosome 17q21. 2. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of epithelial cells structure. Cytokeratins are described to be differentiation markers in several epithelial cancers.

Alternative Names :

CK-23, CK23, Cytokeratin-23, DKFZP434G032, HAIK1, Cytokeratin 23, MGC26158, K23, K1C23, HAIK 1, Histone deacetylase inducible keratin 23, type I cytoskeletal 23

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Birkenkamp-Demtroder K., et al.(2007) Mol Oncol 1(2): 181-195.;; Zhang J.S., et al. (2001) Genes Chromosomes Cancer 30: 123-135.;; Schweizer J., et al. (2006) Cell Biol 174: 169-174.;; Rogers M.A., et al. (2004) Differentiation 72: 527-540.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human KRT23 (271-422aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of KRT23 in Hep3B cells. The cell was stained with ATGA0225 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT2F6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of KRT23 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9C075

Internal Search Terms :

KRT23,ATGA0225-10ug,ATGA0225-20ug,ATGA0225-50ug,ATGA0225-100ug,ATGA0225-250ug,ATGA0225-500ug,ATGA0225-1mg,ATGA0225-10,ATGA0225-20,ATGA0225-50,ATGA0225-100,ATGA0225-250,ATGA0225-500,ATGA0225-1

more info or order :

NKMAX product webpage