Human PNPO antibody | NKMAX ATGA0213 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PNPO antibody

catalog :

ATGA0213

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C7

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PNPO antibody

Catalog number (SKU) :

ATGA0213

NCBI :

NP_060599

Description :

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Alternative Names :

Pyridoxine-5-phosphate oxidase, FLJ10535, PDXPO

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ngo EO, et al. (Jun 1998). Biochemistry 37 (21): 7741-8.;; Mills PB, Surtees RA, et al. (2005). Hum. Mol. Genet. 14 (8): 1077-86.;; Kang JH, et al. (Jun 2004). Eur J Biochem 271 (12): 2452-61.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PNPO (57-261aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,IHC,FACS

IF Comment And Pic :

ICC/IF analysis of PNPO in Hep3B cells. The cell was stained with ATGA0213 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: A549 cell lysate ;;Lane 2.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT2C7

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PNPO in Jurkat cells. The cell was stained with ATGA0213 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

IHC Comment And Pic :

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PNPO (1:150) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine(DAB)

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NVS9

Internal Search Terms :

PNPO,ATGA0213-10ug,ATGA0213-20ug,ATGA0213-50ug,ATGA0213-100ug,ATGA0213-250ug,ATGA0213-500ug,ATGA0213-1mg,ATGA0213-10,ATGA0213-20,ATGA0213-50,ATGA0213-100,ATGA0213-250,ATGA0213-500,ATGA0213-1

more info or order :

NKMAX product webpage