Human EGFR antibody | NKMAX ATGA0212 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EGFR antibody

catalog :

ATGA0212

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2H8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EGFR antibody

Catalog number (SKU) :

ATGA0212

NCBI :

NP_005219

Description :

Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.

Alternative Names :

Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cohen S, (1964) Nat Cancer Inst Monogr, 13: 13-27. ;; Toyoda H, et al. (1995) J Biol Chem, 270: 7495-500. ;; Carpenter G, et al. (1978) Nature,276: 409-10.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human EGFR (424-605aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green).

WB Comment and Pic :

The cell lysates of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT2H8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00533

Internal Search Terms :

EGFR,ATGA0212-10ug,ATGA0212-20ug,ATGA0212-50ug,ATGA0212-100ug,ATGA0212-250ug,ATGA0212-500ug,ATGA0212-1mg,ATGA0212-10,ATGA0212-20,ATGA0212-50,ATGA0212-100,ATGA0212-250,ATGA0212-500,ATGA0212-1

References with this Product :

Hoshi H, et al. Construction of a novel cell-based assay for the evaluation of anti-EGFR drug efficacy against EGFR mutation. (Oncol Rep. 2017){https://pubmed.ncbi.nlm.nih.gov/27840973/}

more info or order :

NKMAX product webpage