Human PKLR antibody | NKMAX ATGA0209 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PKLR antibody

catalog :

ATGA0209

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1E3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PKLR antibody

Catalog number (SKU) :

ATGA0209

NCBI :

NP_000289

Description :

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.

Alternative Names :

Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Meza NW., et al. (2009). Mol Ther. 17(12):2000-9.;; Roy MF., et al. (2007). J Exp Med. 204(12):2949-61.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PKLR (47-574aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PKLR in Hep3B cells. The cell was stained with ATGA0209 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of PKLR in HeLa cells. The cell was stained with ATGA0209 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0209-IF2.jpg}

WB Comment and Pic :

The cell lysate of 293T (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Balb-3T3 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Raji cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: A549 cell lysate {ATGA0209-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1E3

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30613

Internal Search Terms :

PKLR,ATGA0209-10ug,ATGA0209-20ug,ATGA0209-50ug,ATGA0209-100ug,ATGA0209-250ug,ATGA0209-500ug,ATGA0209-1mg,ATGA0209-10,ATGA0209-20,ATGA0209-50,ATGA0209-100,ATGA0209-250,ATGA0209-500,ATGA0209-1

more info or order :

NKMAX product webpage