Human eIF-2 alpha/EIF2S1 antibody | NKMAX ATGA0208 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human eIF-2 alpha/EIF2S1 antibody

catalog :

ATGA0208

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human eIF-2 alpha/EIF2S1 antibody

Catalog number (SKU) :

ATGA0208

NCBI :

NP_004085

Description :

Eukaryotic translation initiation factor 2, subunit 1 alpha (EIF2S1) is plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. The phosphorylation of alpha-subunit of EIF2S1 effectively prevents formation of the EIF2S1/GTP/Met-tRNAi complex and inhibits global protein synthesis.

Alternative Names :

Eukaryotic translation initiation factor 2 subunit alpha, EIF2, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF-2alpha, EIF2A

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kim JH, et al. (2011). EMBO J, 1030(12):2454-64.;; Pena J, et al. (2011). J Biol Chem, 286(16):14226-36.;; Muaddi H, et al. (2010). Mol Biol Cell, 21(18):3220-31.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human EIF2S1 (1-315aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of EIF2S1 in HeLa cells. The cell was stained with ATGA0208 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HeLa cell lysate ;;Lane 2.: Jurkat cell lysate ;;Lane 3.: NIH-3T3 cell lysate

Reacts With :

Human

Clone Number :

AT5E10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P05198

Internal Search Terms :

EIF2S1,ATGA0208-10ug,ATGA0208-20ug,ATGA0208-50ug,ATGA0208-100ug,ATGA0208-250ug,ATGA0208-500ug,ATGA0208-1mg,ATGA0208-10,ATGA0208-20,ATGA0208-50,ATGA0208-100,ATGA0208-250,ATGA0208-500,ATGA0208-1

more info or order :

NKMAX product webpage