Human ARF1 antibody | NKMAX ATGA0207 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ARF1 antibody

catalog :

ATGA0207

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1B3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ARF1 antibody

Catalog number (SKU) :

ATGA0207

NCBI :

NP_001649

Description :

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family member is essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D and cholera toxin. The functions of ARF proteins in membrane traffic and organelle integrity are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. ARF1 is various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane.

Alternative Names :

ADP-ribosylation factor 1, ADP ribosylation factor 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Amor, J. C. et al. (1994) Nature 372: 704-708.;; Claude, A. et al. (1999) J. Cell Biol. 146: 71-84.;; Derrien, V. et al. (2002) J. Cell Sci. 115: 2867-2879.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ARF1 (1-181aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of ARF1 in MCF7 cells. The cell was stained with ATGA0207 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of ARF1 in U87MG cells. The cell was stained with ATGA0207 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0207-IF2.jpg}

WB Comment and Pic :

The cell lysates and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HeLa cell lysate;; Lane 2.: HepG2 cell lysate;; Lane 3.: Brain tissue lysate;; Lane 4.: MCF cell lysate

Reacts With :

Human

Clone Number :

AT1B3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ARF1 in MCF7 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P84077

Internal Search Terms :

ARF1,ATGA0207-10ug,ATGA0207-20ug,ATGA0207-50ug,ATGA0207-100ug,ATGA0207-250ug,ATGA0207-500ug,ATGA0207-1mg,ATGA0207-10,ATGA0207-20,ATGA0207-50,ATGA0207-100,ATGA0207-250,ATGA0207-500,ATGA0207-1

more info or order :

NKMAX product webpage