Human Adenosine Kinase/ADK antibody | NKMAX ATGA0206 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Adenosine Kinase/ADK antibody

catalog :

ATGA0206

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4F8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Adenosine Kinase/ADK antibody

Catalog number (SKU) :

ATGA0206

NCBI :

NP_006712

Description :

Adenosine kinase (ADK) is the key regulator of adenosine metabolism. Because of the manifold receptor-dependent actions of adenosine, tight regulation of adenosine levels is crucial. The intracellular and extracellular pools of adenosine are in dynamic exchange by equilibrative and concentrative nucleoside transporters, so extracellular concentrations of adenosine are regulated by interplay of theses transporters with intracellular and extracellular enzymes of adenosine metabolism. Thus, the extracellular concentration of adenosine is enhanced by inhibition of equilibrative nucleoside transporters such as S- (4-nitrobenzyl) -6-thioinosine and cannabidiol and, stimulation of extracellular ATP breakdown and inhibition of intracellular adenosine removal.

Alternative Names :

ADK, AK, adenosine kinase, adenosine 5'-phosphotransferase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Mathews II , et al. (1998). Biochemistry 1037(45):15607-20.;; Lindberg B, Klenow H, Hansen K (1967). J. Biol. Chem. 242 (3): 350-6.;; CAPUTTO R (1951). J. Biol. Chem. 189 (2): 801-14.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human ADK (22-362aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of ADK in A549 cells. The cell was stained with ATGA0206 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of HepG2 and 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT4F8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ADK in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P55263

Internal Search Terms :

Adenosine Kinase, ADK,ATGA0206-10ug,ATGA0206-20ug,ATGA0206-50ug,ATGA0206-100ug,ATGA0206-250ug,ATGA0206-500ug,ATGA0206-1mg,ATGA0206-10,ATGA0206-20,ATGA0206-50,ATGA0206-100,ATGA0206-250,ATGA0206-500,ATGA0206-1

more info or order :

NKMAX product webpage