Human HPRT antibody | NKMAX ATGA0204 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human HPRT antibody

catalog :

ATGA0204

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G8

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human HPRT antibody

Catalog number (SKU) :

ATGA0204

NCBI :

NP_000185

Description :

HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Alternative Names :

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sculley DG, et al. (1993). Hum. Genet. 90 (3): 195-207.;; Stout JT, Caskey CT (1986). Annu. Rev. Genet. 19: 127-48.;; Davidson BL, et al. (1991). Am. J. Hum. Genet. 48 (5): 951-8.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human HPRT (1-218aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysates of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT2G8

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00492

Internal Search Terms :

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more info or order :

NKMAX product webpage