Human CtBP1 antibody | NKMAX ATGA0199 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CtBP1 antibody

catalog :

ATGA0199

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4D6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CtBP1 antibody

Catalog number (SKU) :

ATGA0199

NCBI :

NP_001319

Description :

The C-terminal-binding protein (CtBP) was originally identified as a cellular protein that interacts with a C-terminal motif of adenovirus E1A oncoproteins. CtBP is highly conserved in higher eukaryotes. Vertebrates contain two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates contain a single CtBP. CtBP family proteins share significant sequence homology with D-2-hydroxy acid dehydrogenases. Also, CtBP proteins associate with various proteins and functions as a co-repressor of transcription.

Alternative Names :

C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Katsanis N, et al. (1998). Genomics 47 (2): 294-9.;; Paliwal S, et al. (March 2006). Mol. Cell. Biol. 26 (6): 2360-72.;; Chinnadurai G (2002). Mol. Cell 9 (2): 213-24.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CTBP1 (1-440aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0199-IF1b.jpg} ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0199-IF2a.jpg} {ATGA0199-IF2b.jpg}

WB Comment and Pic :

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: NIH3T3 cell lysate ;;Lane 3.: 293T cell lysate ;;Lane 4.: AGS cell lysate ;;Lane 5.: Raji cell lysate ;;Lane 6.: A549 cell lysate ;;Lane 7.: Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT4D6

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500. Recommended starting dilution is 1:500

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q13363

Internal Search Terms :

CtBP1, C-terminal-binding protein 1 isoform 1, BARS, C-terminal-binding protein 1 isoform 1 C terminal binding protein 1, CTBP, CTBP1, MGC104684.,ATGA0199-10ug,ATGA0199-20ug,ATGA0199-50ug,ATGA0199-100ug,ATGA0199-250ug,ATGA0199-500ug,ATGA0199-1mg,ATGA0199-10,ATGA0199-20,ATGA0199-50,ATGA0199-100,ATGA0199-250,ATGA0199-500,ATGA0199-1

more info or order :

NKMAX product webpage