Human beta-II Tubulin antibody | NKMAX ATGA0196 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human beta-II Tubulin antibody

catalog :

ATGA0196

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5B2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human beta-II Tubulin antibody

Catalog number (SKU) :

ATGA0196

NCBI :

NP_821080

Description :

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Alternative Names :

TUBB2B, Tubulin beta 2B class Iib, Tubulin beta 2b

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +4C For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

General References :

Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41(6):746-52.;; Bouwmeester T, et al. (2004) Nat Cell Bio, 6(2):97-105.;; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42(4): 315-330.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human beta-Tubulin (1-445aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red).Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green).{ATGA0196-IF2.jpg}Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).{ATGA0196-IF3.jpg}

WB Comment and Pic :

The cell lysates(40ug) and tissue lysates(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: A549 cell lysate;;Lane 3.: A431 cell lysate;;Lane 4.: HepG2 cell lysate;;Lane 5.: Mouse brain tissue lyate

Reacts With :

Human

Clone Number :

AT5B2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction, Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9BVA1

Internal Search Terms :

b-Tubulin, DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, bA506K6.1, TUBB2B, Tubulin beta 2b, Tubulin beta-2B,ATGA0196-10ug,ATGA0196-20ug,ATGA0196-50ug,ATGA0196-100ug,ATGA0196-250ug,ATGA0196-500ug,ATGA0196-1mg,ATGA0196-10,ATGA0196-20,ATGA0196-50,ATGA0196-100,ATGA0196-250,ATGA0196-500,ATGA0196-1

more info or order :

NKMAX product webpage