Human NQO2 antibody | NKMAX ATGA0192 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NQO2 antibody

catalog :

ATGA0192

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1E3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NQO2 antibody

Catalog number (SKU) :

ATGA0192

NCBI :

AAH06096

Description :

Quinone oxidoreductase (NQO1 and NQO2) are cytosolic proteins that catalyze metabolic redaction of quinines and derivates. NQO2 is inhibited by flavones such as quercetin. Also benzo (a) pyrene is another known inhibitor of NQO2. Even though overlapping substrates specificities have been observed for NQO1 and NQO2, such as for CB1954 activation, significant differences exist in relative affinities for the various substrates. The detoxification role of NQO2 has not been found, and it has no known endogenous biological substrates. However, NQO1 plays an important role in the detoxification of various endogenous and exogenous quinones, including estrogen quinines. Also NQO2 has a melatonin-binding site, which may explain the anti-oxidant role of melatonin related with circadian rhythm.

Alternative Names :

N-ribosyldihydronicotinamide:quinone reductase 2, NMOR2, NAD(P)H menadione oxidoreductase 2, Dioxin-inducible, NAD(P)H quinone dehydrogenase 2, Quinone reductase 2, QR2, DHQV, DIA6, NRH:quinone oxidoreductase 2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wu K., et al,. (1997) Arch Biochem Biophys. 347(2):221-8.;; Aiswal AK., et al,. (1994) J Biol Chem. 269(20):14502-8.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NQO2 (1-231aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of NQO2 in A549 cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of NQO2 in HeLa cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0192-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: HeLa cell lysate;; Lane 2.: K562 cell lysate;; Lane 3.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT1E3

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P16083

Internal Search Terms :

NQO2,ATGA0192-10ug,ATGA0192-20ug,ATGA0192-50ug,ATGA0192-100ug,ATGA0192-250ug,ATGA0192-500ug,ATGA0192-1mg,ATGA0192-10,ATGA0192-20,ATGA0192-50,ATGA0192-100,ATGA0192-250,ATGA0192-500,ATGA0192-1

more info or order :

NKMAX product webpage