Human beta-II Tubulin antibody | NKMAX ATGA0187 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human beta-II Tubulin antibody

catalog :

ATGA0187

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5B3

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human beta-II Tubulin antibody

Catalog number (SKU) :

ATGA0187

NCBI :

NP_821080

Description :

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mainly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. Also, TUBB2B is a new critical gene involved in neuronal migration. It is present in the nuclei and nucleoplasm, Defects in this gene are a cause of asymmetric polymicrogyria.

Alternative Names :

TUBB2B, Tubulin beta 2B class Iib, Tubulin beta 2b

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41(6):746-52.;; Bouwmeester T, et al. (2004) Nat Cell Bio, 6(2):97-105.;; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42(4): 315-330.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human beta-Tubulin (1-445aa) purified from E. coli

Applications :

ELISA, WB, IHC

WB Comment and Pic :

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse brain tissue lysate

Reacts With :

Human

Clone Number :

AT5B3

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human beta-Tubulin antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9BVA1

Internal Search Terms :

b-Tubulin, bA506K6.1, DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, tubulin beta 2B,ATGA0187-10ug,ATGA0187-20ug,ATGA0187-50ug,ATGA0187-100ug,ATGA0187-250ug,ATGA0187-500ug,ATGA0187-1mg,ATGA0187-10,ATGA0187-20,ATGA0187-50,ATGA0187-100,ATGA0187-250,ATGA0187-500,ATGA0187-1

more info or order :

NKMAX product webpage