Human Aurora B antibody | NKMAX ATGA0185 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Aurora B antibody

catalog :

ATGA0185

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2B1

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Aurora B antibody

Catalog number (SKU) :

ATGA0185

NCBI :

NP_004208

Description :

The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells.

Alternative Names :

Serine/threonine-protein kinase 12, Aurora-B, AIK2, AIM1, ARK2, AurB, aurkb-sv1, aurkb-sv2, IPL1, STK12, aurkb, AIM1, AuRKB, Aurora 1, STK1, STK5, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, Serine/theronine kinase 12, Serine/threonine protein kinase 12

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Posch M, et al. (2010) J Cell Biol, 191(1):61-74.;; Lisa L, et al. (2009) J Cell Biol, 186(4): 491-507.;; S L Smith, et al. (2005) Br J Cancer, 93(6): 719-729.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Aurora kinase B (1-344aa) purified from E. coli

Applications :

ELISA,WB,FACS

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Aurora kinase B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant Human Aurora kinase A;;Lane 2.: Recombinant Human Aurora kinase B

Reacts With :

Human

Clone Number :

AT2B1

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Aurora B in LNCap cells. The cell was stained with ATGA0185 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96GD4

Internal Search Terms :

Aurora B,ATGA0185-10ug,ATGA0185-20ug,ATGA0185-50ug,ATGA0185-100ug,ATGA0185-250ug,ATGA0185-500ug,ATGA0185-1mg,ATGA0185-10,ATGA0185-20,ATGA0185-50,ATGA0185-100,ATGA0185-250,ATGA0185-500,ATGA0185-1

more info or order :

NKMAX product webpage