Human BAK antibody | NKMAX ATGA0183 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human BAK antibody

catalog :

ATGA0183

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT38E2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human BAK antibody

Catalog number (SKU) :

ATGA0183

NCBI :

NP_001179

Description :

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

Alternative Names :

BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3(2): 116-118.;; Libo Shan, et al. (2008) Cell Host Microbe, 4(1): 17-27.;; Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104(50): 19983-19988.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human BAK1 (29-187aa) purified from E.coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of BAK1 in HeLa cells. The cell was stained with ATGA0183 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1 : 293T cell lysate;; Lane 2 : HeLa cell lysate;; Lane 3 : A431 cell lysate;; Lane 4 : A549 cell lysate;; Lane 5 : Jurkat cell lysate;; Lane 6 : MCF7 cell lysate;; Lane 7 : PC3 cell lysate

Reacts With :

Human

Clone Number :

AT38E2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q16611

Internal Search Terms :

BAK,ATGA0183-10ug,ATGA0183-20ug,ATGA0183-50ug,ATGA0183-100ug,ATGA0183-250ug,ATGA0183-500ug,ATGA0183-1mg,ATGA0183-10,ATGA0183-20,ATGA0183-50,ATGA0183-100,ATGA0183-250,ATGA0183-500,ATGA0183-1

more info or order :

NKMAX product webpage