Human ERK2/MAPK1 antibody | NKMAX ATGA0182 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ERK2/MAPK1 antibody

catalog :

ATGA0182

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ERK2/MAPK1 antibody

Catalog number (SKU) :

ATGA0182

NCBI :

NP_620407

Description :

Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs). It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.

Alternative Names :

PRKM2, PRKM1, p42MAPK, p41MAPK, p41, p40, p38, Mitogen-activated protein kinase 1 MAP kinase 2, Mitogen-activated protein kinase 1, MAPK2, MAPK1, Extracellular Signal Regulated Kinase 2, ERT1, ERK2, ERK, ,

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107(5):2037-42.;; Gesslein B, et al. (2010) Mol Vis,16:392-407.;; Lai EW, et al. (2004) J Invest Surg, 17:45-53.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human MAPK1 (1-360) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).

WB Comment and Pic :

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT1A4

Ab Usage :

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000. Recommended starting dilution is 1:500

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P28482

Internal Search Terms :

ERK, ERK 2, ERK2, ERT 1, ERT1, Extracellular Signal Regulated Kinase 2, MAPK 1, MAPK 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1 MAP kinase 2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, ATGA0182-10ug, ATGA0182-20ug, ATGA0182-50ug, ATGA0182-100ug, ATGA0182-250ug, ATGA0182-500ug, ATGA0182-1mg, ATGA0182-010, ATGA0182-020, ATGA0182-050, ATGA0182-100, ATGA0182-250, ATGA0182-500, ATGA0182-01M

more info or order :

NKMAX product webpage