antibody

Human GAPDH antibody

ATGA0181

50 ul

170 USD

monoclonal

mouse

nonconjugated

AT8G4

western blot, ELISA, immunocytochemistry

Human GAPDH antibody

ATGA0181

NP_002037

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Chuang DM, et al. (2005) Annu Rev Pharmacol Toxicol, 45:269-90.;; Mazzola JL, et al. (2002) Neurotoxicology, 23(4-5):603-9.;; Sirover MA. (1997) J Cell Biochem, 66(2):133-40.

Mouse

IgG2b kappa

Recombinant human GAPDH (1-335aa) purified from E.coli

ELISA, WB, ICC/IF

Immunofluorescence of human HeLa cells stained with Hoechst 3342(Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red

The HeLa cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Anti-human GAPDH antibody(1:1000);;Lane 2.: Anti-human GAPDH antibody(1:2000);;Lane 3.: Anti-human GAPDH antibody(1:4000);;Lane 4.: Anti-human GAPDH antibody(1:6000);;Lane 5.: Anti-human GAPDH antibody(1:8000);;Lane 6.: Anti-human GAPDH antibody(1:10000);;

Human

AT8G4

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Human

Cancer

Unconjugated

Monoclonal

By protein-G affinity chromatography

Monoclonal Antibody

P04406

wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

Migas AA, et al. RUNX1T1/MTG8/ETO gene expression status in human t(8;21)(q22;q22)-positive acute myeloid leukemia cells. (Leuk Res. 2014){https://pubmed.ncbi.nlm.nih.gov/24976338/};;Yu H, et al. Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor. (Oncol Rep. 2019){https://pubmed.ncbi.nlm.nih.gov/31173262/}