Human Lactate Dehydrogenase A/LDHA antibody | NKMAX ATGA0180 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Lactate Dehydrogenase A/LDHA antibody

catalog :

ATGA0180

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Lactate Dehydrogenase A/LDHA antibody

Catalog number (SKU) :

ATGA0180

NCBI :

NP_005557

Description :

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Alternative Names :

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107(5):2037-42.;; Xie H, et al. (2009) Mol Cancer Ther, 8(3):626-35.;; Toyoda K, et al. (2010) J Bacteriol, 191(13):4251-8.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human LDHA (1-332aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: 293T cell lysate;;Lane 3.: A431 cell lysate;;Lane 4.: HeLa cell lysate;;

Reacts With :

Human

Clone Number :

AT1A4

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00338

Internal Search Terms :

Lactate Dehydrogenase A, LDHA,ATGA0180-10ug,ATGA0180-20ug,ATGA0180-50ug,ATGA0180-100ug,ATGA0180-250ug,ATGA0180-500ug,ATGA0180-1mg,ATGA0180-10,ATGA0180-20,ATGA0180-50,ATGA0180-100,ATGA0180-250,ATGA0180-500,ATGA0180-1

more info or order :

NKMAX product webpage