Human OAT antibody | NKMAX ATGA0176 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human OAT antibody

catalog :

ATGA0176

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT23A2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human OAT antibody

Catalog number (SKU) :

ATGA0176

NCBI :

NP_000265

Description :

OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

Alternative Names :

ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Stranska J, et al. (2008) Plant Signal Behav, 3(11):929-35.;; Canas RA, et al. (2008) Plant Physiol, 148(1):77-88.;; Levillain O, et al. (2007) Am J Physiol Renal Physiol, 292(3):F1016-27.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human OAT (33-439aa) purified from E.coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of OAT in HeLa cells. The cell was stained with ATGA0176 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of OAT in Hep3B cells. The cell was stained with ATGA0176 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0176-IF2.jpg}

WB Comment and Pic :

Tissue lysate of mouse liver (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant protein (20ng) and lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OAT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: OAT recombinant protein;;Lane 2.: 293T cell lysate;;Lane 3.: Mouse brain tissue lysate {ATGA0176-WB2.jpg}

Reacts With :

Human

Clone Number :

AT23A2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P04181

Internal Search Terms :

OAT,ATGA0176-10ug,ATGA0176-20ug,ATGA0176-50ug,ATGA0176-100ug,ATGA0176-250ug,ATGA0176-500ug,ATGA0176-1mg,ATGA0176-10,ATGA0176-20,ATGA0176-50,ATGA0176-100,ATGA0176-250,ATGA0176-500,ATGA0176-1

more info or order :

NKMAX product webpage