Human Thymidylate synthase/TYMS antibody | NKMAX ATGA0169 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Thymidylate synthase/TYMS antibody

catalog :

ATGA0169

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT21S5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Thymidylate synthase/TYMS antibody

Catalog number (SKU) :

ATGA0169

NCBI :

NP_001062

Description :

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in activity highest proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.

Alternative Names :

HST422, TMS, TS, d TMP synthase, EC 2.1.1.45, MGC88736, Thymidylate synthase, Thymidylate synthetase, Tsase, TYMS, TYMS protein, Tyms thymidylate synthetase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shahrokni A, et al., (2009) Clin Colorectal Cancer 8(4):231-4.;; Henriquez-Hernandez LA, et al., (2009) Oncol Rep 22(6):1425-33.;; Colavito D, et al., (2009) Cancer Chemother Pharmacol 64(6):1195-200.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant TYMS (1-313aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

HeLa cells were stained with monoclonal anti-TYMS antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). ICC/IF analysis of TYMS in HeLa cells. The cell was stained with ATGA0169 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0169-IF2.jpg}

WB Comment and Pic :

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TYMS (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: Recombinant human TYMS protein ;;Lane 2.: HeLa cell lysate ;;Lane 3.: Jurkat cell lysate ;;Lane 4.: MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT21S5

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TYMS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P04818

Internal Search Terms :

TYMS,ATGA0169-10ug,ATGA0169-20ug,ATGA0169-50ug,ATGA0169-100ug,ATGA0169-250ug,ATGA0169-500ug,ATGA0169-1mg,ATGA0169-10,ATGA0169-20,ATGA0169-50,ATGA0169-100,ATGA0169-250,ATGA0169-500,ATGA0169-1

more info or order :

NKMAX product webpage