Human E2-EPF/UBE2S antibody | NKMAX ATGA0165 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human E2-EPF/UBE2S antibody

catalog :

ATGA0165

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human E2-EPF/UBE2S antibody

Catalog number (SKU) :

ATGA0165

NCBI :

NP_055316.2

Description :

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Alternative Names :

Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10(11):755-64.;; Windheim M, et al. (2008) Biochem J, 409(3):723-9.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human UBE2S (1-222aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).

WB Comment and Pic :

The cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: UBE2S Transfected 293T cell lysate;;

Reacts With :

Human

Clone Number :

AT2C12

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of UBE2S in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q16763

Internal Search Terms :

UBE2S,ATGA0165-10ug,ATGA0165-20ug,ATGA0165-50ug,ATGA0165-100ug,ATGA0165-250ug,ATGA0165-500ug,ATGA0165-1mg,ATGA0165-10,ATGA0165-20,ATGA0165-50,ATGA0165-100,ATGA0165-250,ATGA0165-500,ATGA0165-1

more info or order :

NKMAX product webpage