Human CRABP1 antibody | NKMAX ATGA0162 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CRABP1 antibody

catalog :

ATGA0162

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CRABP1 antibody

Catalog number (SKU) :

ATGA0162

NCBI :

NP_004369

Description :

CRABP1 (cellular retinoic acid-binding protein 1) is a member of specific carrier proteins for members of the vitamin A family. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. CRABP1 is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. It is constitutively expressed and is believed to have different functions in the cell than the related CRABP2. Interestingly, Sytenol A bakuchiol has very specific receptor specificity over retinol and has no effect on the RAR-beta and RAR-gamma receptors and down-regulates CRABP1.

Alternative Names :

Cellular retinoic acid binding protein 1, CRABP, CRABP-I, CRABPI, RBP5, Cellular retinoic acid binding protein 1 CRABP 1, CRABP I, RBP 5, Retinoic acid binding protein I cellular

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wang L, et al. (1997) J Biol Chem, 272(3):1541-7.;;. Nezzar H, et al. (2007) Mol Vis, 13:1641-50.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CRABP1 (1-137aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CRABP1 in Balb/3T3 cells. The cell was stained with ATGA0162 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Eye Tissue lysateThe cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;{2004010134347560.jpg}The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: CRABP1 Transfected 293T cell lysate;;{2004010134867354.jpg}

Reacts With :

Human

Clone Number :

AT1A1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P29762

Internal Search Terms :

CRABP1,ATGA0162-10ug,ATGA0162-20ug,ATGA0162-50ug,ATGA0162-100ug,ATGA0162-250ug,ATGA0162-500ug,ATGA0162-1mg,ATGA0162-10,ATGA0162-20,ATGA0162-50,ATGA0162-100,ATGA0162-250,ATGA0162-500,ATGA0162-1

more info or order :

NKMAX product webpage