Human G6PD antibody | NKMAX ATGA0158 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human G6PD antibody

catalog :

ATGA0158

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human G6PD antibody

Catalog number (SKU) :

ATGA0158

NCBI :

NP_001035810.1

Description :

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.

Alternative Names :

glucose-6-phosphate 1-dehydrogenase, G6PD1, G6PDH

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Huang Y., et al, (2008) Mol Genet Metab. 93(1):44-53.;; Zimny A., et al. (2003) Pol Arch Med Wewn. 110(5):1327-33.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human G6PD (35-506aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of G6PD in HeLa cells. The cell was stained with ATGA0158 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human G6PD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Jurkat cell lysate;;

Reacts With :

Human

Clone Number :

AT2F6

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P11413

Internal Search Terms :

G6PD,ATGA0158-10ug,ATGA0158-20ug,ATGA0158-50ug,ATGA0158-100ug,ATGA0158-250ug,ATGA0158-500ug,ATGA0158-1mg,ATGA0158-10,ATGA0158-20,ATGA0158-50,ATGA0158-100,ATGA0158-250,ATGA0158-500,ATGA0158-1, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p

more info or order :

NKMAX product webpage