Human TDP1 antibody | NKMAX ATGA0157 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TDP1 antibody

catalog :

ATGA0157

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1F2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TDP1 antibody

Catalog number (SKU) :

ATGA0157

NCBI :

NP_060789

Description :

Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.

Alternative Names :

tyrosyl-DNA phosphodiesterase 1, FLJ11090, SCAN1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dexheimer TS, et al., (2008) Anticancer Agents Med Chem. 8(4):381-9.;; EI-Khamisy SF, Caldecott KW., (2006) Mutagenesis. 21(4):219-24.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human TDP1 (1-298aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of TDP1 in Hep3B cells. The cell was stained with ATGA0157 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of MCF7(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TDP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;

Reacts With :

Human

Clone Number :

AT1F2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TDP1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NUW8

Internal Search Terms :

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more info or order :

NKMAX product webpage