Human TBCA antibody | NKMAX ATGA0155 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TBCA antibody

catalog :

ATGA0155

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TBCA antibody

Catalog number (SKU) :

ATGA0155

NCBI :

NP_004598

Description :

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.

Alternative Names :

Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nolasco S, et al., (2005) FEBS Lett 579(17):3515-3524.;; Tian G, et al., (1996) Cell 86(2):287-296.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant TBCA (1-108aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of TBCA in A549 cells. The cell was stained with ATGA0155 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of TBCA in HeLa cells. The cell was stained with ATGA0155 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010129463677.jpg}

WB Comment and Pic :

The cell lysate of LNCap(20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: K562 cell lysate;;{ATGA0155-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1A5

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TBCA in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O75347

Internal Search Terms :

TBCA,ATGA0155-10ug,ATGA0155-20ug,ATGA0155-50ug,ATGA0155-100ug,ATGA0155-250ug,ATGA0155-500ug,ATGA0155-1mg,ATGA0155-10,ATGA0155-20,ATGA0155-50,ATGA0155-100,ATGA0155-250,ATGA0155-500,ATGA0155-1

more info or order :

NKMAX product webpage