antibody

Human MYL2 antibody

ATGA0151

50 ul

170 USD

monoclonal

mouse

nonconjugated

AT3B2

western blot, ELISA, immunocytochemistry, FACS

Human MYL2 antibody

ATGA0151

NP_000423

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy

Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Macera MJ, et al., (1992) Genomics. 13(3):829-31.;; Poetter K, et al., (1996) Nat Genet. 13(1):63-9.

Mouse

IgG2b kappa

Recombinant human MYL2 (1-166aa) purified from E. coli

ELISA, WB, ICC/IF, FACS

ICC/IF analysis of MYL2 in A431 cells. The cell was stained with ATGA0151 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse heart tissue lysate

Human

AT3B2

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of MYL2 in A431 cells. The cell was stained with ATGA0151 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Human

Cytoskeleton

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal Antibody

P10916

MYL2,ATGA0151-10ug,ATGA0151-20ug,ATGA0151-50ug,ATGA0151-100ug,ATGA0151-250ug,ATGA0151-500ug,ATGA0151-1mg,ATGA0151-10,ATGA0151-20,ATGA0151-50,ATGA0151-100,ATGA0151-250,ATGA0151-500,ATGA0151-1

Beveridge RD, et al. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling. (Cell Cycle. 2014){https://pubmed.ncbi.nlm.nih.gov/25485589/};;Russo V, et al. Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture. (Biores Open Access. 2015){https://pubmed.ncbi.nlm.nih.gov/26487982/};;Mourino-Alvarez L, et al. A comprehensive study of calcific aortic stenosis: from rabbit to human samples. (Dis Model Mech. 2018){https://pubmed.ncbi.nlm.nih.gov/29752279/};;Acharya BR, et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. (Dev Cell. 2018){https://pubmed.ncbi.nlm.nih.gov/30318244/}