Human Casein kinase 1 alpha/CSNK1A1 antibody | NKMAX ATGA0149 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Casein kinase 1 alpha/CSNK1A1 antibody

catalog :

ATGA0149

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2E2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Casein kinase 1 alpha/CSNK1A1 antibody

Catalog number (SKU) :

ATGA0149

NCBI :

NP_001883

Description :

Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex

Alternative Names :

Casein kinase 1 alpha 1, CK1, CK1a, CK1alpha, CKIa, CKIalpha, Clock regulator kinase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Knippschild U, et al., (2005) Onkologie. 28(10):508-14.;; Katoh M., (2007) Clin Cancer Res. 13(14):4042-5.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of Casein Kinase 1 alpha in HeLa cells. The cell was stained with ATGA0149 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of HeLa and MCF7(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein Kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein Kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;{ATGA0149-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2E2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P48729

Internal Search Terms :

Casein Kinase1 a,ATGA0149-10ug,ATGA0149-20ug,ATGA0149-50ug,ATGA0149-100ug,ATGA0149-250ug,ATGA0149-500ug,ATGA0149-1mg,ATGA0149-10,ATGA0149-20,ATGA0149-50,ATGA0149-100,ATGA0149-250,ATGA0149-500,ATGA0149-1

more info or order :

NKMAX product webpage