Aequorea victoria GFP antibody | NKMAX ATGA0148 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Aequorea victoria GFP antibody

catalog :

ATGA0148

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G5

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Aequorea victoria GFP antibody

Catalog number (SKU) :

ATGA0148

Description :

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Alternative Names :

Green fluorescent protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Phillips G (2001) FEMS Microbiol Lett 204(1):9-18.;;Tsien R (1998) Annu Rev Biochem 67:509-544.;;Prasher, et al., (1992) Gene 111(2):229-233.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant GFP (1-238aa) purified from E. coli

Applications :

ELISA, WB

WB Comment and Pic :

The recombinant proteins were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human GFP protein (20ng);;Lane 2.: Recombinant human GFP protein (40ng);;Lane 3.: Recombinant human GFP protein (80ng)The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: GFP transfected 293T cell lysate {ATGA0148-WB2.jpg}

Reacts With :

Aequorea victoria

Clone Number :

AT2G5

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

GFP

Gene Category :

Fusion Tag & Detection

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P42212

Internal Search Terms :

GFP,ATGA0148-10ug,ATGA0148-20ug,ATGA0148-50ug,ATGA0148-100ug,ATGA0148-250ug,ATGA0148-500ug,ATGA0148-1mg,ATGA0148-10,ATGA0148-20,ATGA0148-50,ATGA0148-100,ATGA0148-250,ATGA0148-500,ATGA0148-1

more info or order :

NKMAX product webpage