Human ORF73/HHV8 antibody | NKMAX ATGA0145 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ORF73/HHV8 antibody

catalog :

ATGA0145

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4C11

reactivity :

human

application :

western blot, ELISA, immunohistochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ORF73/HHV8 antibody

Catalog number (SKU) :

ATGA0145

NCBI :

AAB62657

Description :

ORF73 protein of human herpesvirus 8 (HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.

Alternative Names :

Human herpesvirus 8 ORF73, Kaposi's sarcoma-associated herpesvirus

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

kedes D.H., et al. (1997) Journal of Clinical Investigation. 100: 2610-2606.;; Verma SC., et al (2006) Journal of Virology 80: 3445-3458.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli

Applications :

ELISA,WB,IHC

WB Comment and Pic :

The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ORF73/HHV8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate (40ug);;Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate (40ug);;Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug){ATGA0145-WB2.jpg}

Reacts With :

Human

Clone Number :

AT4C11

Ab Usage :

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of Kaposi's sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Neuroscience

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O40947

Internal Search Terms :

ORF73, HHV8,ATGA0145-10ug,ATGA0145-20ug,ATGA0145-50ug,ATGA0145-100ug,ATGA0145-250ug,ATGA0145-500ug,ATGA0145-1mg,ATGA0145-10,ATGA0145-20,ATGA0145-50,ATGA0145-100,ATGA0145-250,ATGA0145-500,ATGA0145-1

more info or order :

NKMAX product webpage