Human Epoxide Hydrolase 1/EPHX1 antibody | NKMAX ATGA0143 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Epoxide Hydrolase 1/EPHX1 antibody

catalog :

ATGA0143

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2E5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Epoxide Hydrolase 1/EPHX1 antibody

Catalog number (SKU) :

ATGA0143

NCBI :

NP_000111

Description :

Epoxide hydrolase 1 (EPHX1) is a family of biotransformation enzyme which is involved in the hydrolysis of various epoxides and epoxide intermediates. Epoxide hydrolase plays an important role in both the activation and detoxification of exogenous chemicals such as polycyclic aromatic hydrocarbons.

Alternative Names :

Epoxide hydrolase 1, Epoxide hydrolase 1 microsomal (xenobiotic), MEH, EPHX, EPOX

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bertvan Loo, et al., (2006) Appl Environ Microbiol 72(4):2905-2917.;; Janet K.C, et al., (2001) The Histochemical Journal 33:329-336.;; Hassett C, et al., (1994) Genomics 23(2):433-442.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human EPHX1 (21-455aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of EPHX1 in Hep3B cells. The cell was stained with ATGA0143 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Liver Tissue lysateThe cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: EPHX1 Transfected 293T cell lysate;;{ATGA0143-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2E5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07099

Internal Search Terms :

Microsomal (xenobiotic), MEH, Epoxide hydrolase 1 microsomal (xenobiotic), Epoxide hydrolase 1, EPOX, EPHX1, EPHX, ATGA0143-10ug, ATGA0143-20ug, ATGA0143-50ug, ATGA0143-100ug, ATGA0143-250ug, ATGA0143-500ug, ATGA0143-1mg, ATGA0143-010, ATGA0143-020, ATGA0143-050, ATGA0143-100, ATGA0143-250, ATGA0143-500, ATGA0143-01M

more info or order :

NKMAX product webpage