Human Retinol Binding Protein 4/RBP4 antibody | NKMAX ATGA0136 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Retinol Binding Protein 4/RBP4 antibody

catalog :

ATGA0136

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2B4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Retinol Binding Protein 4/RBP4 antibody

Catalog number (SKU) :

ATGA0136

NCBI :

NP_006735

Description :

Retinol binding protein 4 (RBP4) belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.

Alternative Names :

Plasma retinol-binding protein, PRBP, RBP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tan Bk, et al., (2007) J Clin Endocrinol Metab 92(7):2590-2597.;; Yao-Borengasser A, et al., (2007) J Clin Endocrinol Metab 92(7):2430-2432.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human RBP4 (19-201aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The HepG2 cell lysate (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RBP4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RBP4 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: RBP4 Transfected 293T cell lysate;;{ATGA0136-WB2.jpg}

Reacts With :

Human

Clone Number :

AT2B4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of RBP4 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P02753

Internal Search Terms :

RBP4, Retinol-Binding Protein4,ATGA0136-10ug,ATGA0136-20ug,ATGA0136-50ug,ATGA0136-100ug,ATGA0136-250ug,ATGA0136-500ug,ATGA0136-1mg,ATGA0136-10,ATGA0136-20,ATGA0136-50,ATGA0136-100,ATGA0136-250,ATGA0136-500,ATGA0136-1

more info or order :

NKMAX product webpage