Human 14-3-3 theta antibody | NKMAX ATGA0132 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human 14-3-3 theta antibody

catalog :

ATGA0132

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1A1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human 14-3-3 theta antibody

Catalog number (SKU) :

ATGA0132

NCBI :

NP_006817

Description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.

Alternative Names :

YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta, Protein theta

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Liu YC, et al., (1996) J Biol Chem 271:14591-14595.;; Xiao B, et al., (1995) Nature 376:188-191.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human 14-3-3 tau (1-245aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of 14-3-3 tau in HeLa cells. The cell was stained with ATGA0132 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of Jurkat (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : Recombinant Human YWHAZ;; Lane 2. : Recombinant Human YWHAB ;;Lane 3. : Recombinant Human YWHAE ;;Lane 4. : Recombinant Human YWHAH;;Lane 5. : Recombinant Human YWHAG;;Lane 6. : Recombinant Human SFN ;;Lane 7. : Recombinant Human YWHAQ;; {ATGA0132-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : MDA-MB-21 cell lysate ;;Lane 2. : HeLa cell lysate ;;Lane 3. : A431 cell lysate ;;Lane 4. : NIH3T3 cell lysate ;;Lane 5. : 293T cell lysate ;;Lane 6. : HepG2 cell lysate ;;{ATGA0132-WB3.jpg}

Reacts With :

Human

Clone Number :

AT1A1

Ab Usage :

The antibody has been tested by ELISA, Western blot, and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P27348

Internal Search Terms :

1433 theta,ATGA0132-10ug,ATGA0132-20ug,ATGA0132-50ug,ATGA0132-100ug,ATGA0132-250ug,ATGA0132-500ug,ATGA0132-1mg,ATGA0132-10,ATGA0132-20,ATGA0132-50,ATGA0132-100,ATGA0132-250,ATGA0132-500,ATGA0132-1

more info or order :

NKMAX product webpage