Human KLF4 antibody | NKMAX ATGA0129 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human KLF4 antibody

catalog :

ATGA0129

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4E6

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human KLF4 antibody

Catalog number (SKU) :

ATGA0129

NCBI :

NP_004226.3

Description :

kLF4, which is also known as the gut-enriched kruppel-like factor (GkLF), is a zinc finger-containing transcription factor that belongs to the kruppel-like family of transcription factors. kLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. kLF4 is also involved in cell cycle control since expression of kLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

Alternative Names :

krueppel-like factor 4, EZF, GKLF

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yan FF, et al., (2008) Med Hypotheses. 70(4):845-7.;; Cho YG, et al., (2007) APMIS. 115(7):802-8.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human KLF4 (1-170aa) purified from E. coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human KLF4 protein

Reacts With :

Human

Clone Number :

AT4E6

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of KLF4 in A549 cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{ATGA0129-FC2.jpg}

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O43474

Internal Search Terms :

KLF4,ATGA0129-10ug,ATGA0129-20ug,ATGA0129-50ug,ATGA0129-100ug,ATGA0129-250ug,ATGA0129-500ug,ATGA0129-1mg,ATGA0129-10,ATGA0129-20,ATGA0129-50,ATGA0129-100,ATGA0129-250,ATGA0129-500,ATGA0129-1

References with this Product :

Petrova A, et al. Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin. (Stem Cells Dev. 2016){https://pubmed.ncbi.nlm.nih.gov/27460132/}

more info or order :

NKMAX product webpage